Figure 5 | Scientific Reports

Figure 5

From: Dual non-contiguous peptide occupancy of HLA class I evoke antiviral human CD8 T cell response and form neo-epitopes with self-antigens

Figure 5

Presence of T cells activated by combination of short peptides from self and non-self antigens. (a) List of N-terminal 5mer peptides from endogenous self-protein known to bind HLA-A*11:01 that were used for refolding screening. (b) Native western blot screening results indicate formation of stable pHLA complexes from refolded HLA-A*11:01 heavy chain, β2 m and EBV LMP2 C-terminal 5mer peptide (CPLSK) together with three self-peptides (S4:SSSPQ, S15:KICMQ and S21:KTYGE). (c) Detection of CD8+ T cells that recognize self and non-self combinatorial epitope in 14-day PBMC culture of a representative HLA-A*11:01 homozygous individual after stimulation with endogenous self 5mer N-terminal peptides (S4, S15 and S21), CPLSK peptide or the combinatorial self and non-self peptides.

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