Figure 4 | Scientific Reports

Figure 4

From: Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo

Figure 4

Gene expression profiles in WT and MsrB1 KO BMDMs. (A) Relative amounts of mRNA encoding LPS-inducible cytokines, chemokines, and other inflammatory mediators in WT and MsrB1 KO BMDMs were measured after the indicated durations of LPS stimulation. Tnf (TNF), Cxcl1 (CXCL1, GRO1), Csf2 (GM-CSF), Ifnb1 (IFN-β), Dusp1 (DUSP1), Cxcl2 (CXCL2, GRO2, MIP-2α), Il12b (IL-12B, IL-12p40), Il12a (IL-12A, IL-12p35), Il1b (IL-1β), Il1a (IL-1α), Ccl3 (CCL3, MIP-1α), Il6 (IL-6), Ptgs2 (PTGS2, COX-2), Cxcl10 (CXCL10, IP-10), Edn1 (ET-1, PPET1), Ccl4 (CCL4), Nos2 (NOS2, iNOS), Ch25h (h25OH), Il10 (IL-10), Il1rn (IL-1RA) were analyzed. (B–E) Relative mRNA amounts for Il10 (B), Il1rn (C), Il12a (D), and Il12b (E) were measured as in (A). Data is presented as percent values relative to the mRNA amounts at four hours after LPS treatment in WT BMDMs. Error bars represent standard deviation. The experiments were independently repeated three times. Data were statistically analyzed using Student’s t-test (p < 0.01).

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