Figure 1 | Scientific Reports

Figure 1

From: PKC-ѳ is dispensable for OX40L-induced TCR-independent Treg proliferation but contributes by enabling IL-2 production from effector T-cells

Figure 1

OX40-KO Tregs show impaired proliferation compared to WT Tregs. WT and OX40−/−splenic total CD4+ T-cells, isolated and labelled with CellTrace violet, were co-cultured with WT-BMDCs. After five days, cells were harvested and analysed by flow cytometry. (a) Dot plot (left) and summarizing bar graphs (right) show Treg proliferation in OX40-KO cells compared to WT. (b) Dot plot (left) and summarizing bar graphs (right) showing the expression of Foxp3 and OX40 in WT and OX40-KO CD4+ T-cells.  (c) Dot plot (left) and summarizing bar graphs (right) showing the expression of OX40 among the proliferating and resting Tregs (CD4+Foxp3+). Values show average ± SD, *P < 0.05, **p < 0.005, and ***p < 0.0005.

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