Figure 4 | Scientific Reports

Figure 4

From: PKC-ѳ is dispensable for OX40L-induced TCR-independent Treg proliferation but contributes by enabling IL-2 production from effector T-cells

Figure 4

PKC-Ѳ-KO total CD4+ T-cells when co-cultured with WT-BMDCs showed a defect in Treg proliferation. (a,b) Total CD4+ T-cells were isolated from the spleens of WT and PKC-Ѳ-KO mice, CellTrace violet-labelled, then co-cultured with WT-BMDCs. Cells were harvested on day 5 and analysed by flow cytometry for Treg proliferation. (a) Representative dot plots (b) summary bar graphs of percentage of proliferating Tregs. (c,d) Splenic CD4+CD25+ T-cells isolated from WT, PKCѲ-KO and OX40-KO mice, then cultured in the presence of exogenous IL-2, with microbeads coated with anti-CD3/anti-CD28 or WT/Class-II-KO-BMDCs. Cells were harvested after 4 days and analysed by flow cytometry. Dot plots (c) showing the proliferation of Tregs and bar graphs (d) representative of data shown in (c). Numbers shown in the dot plot is the average percentage (n = 3). Values show average ± SD, *P < 0.05, **p < 0.005, and ***p < 0.0005.

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