Figure 5 | Scientific Reports

Figure 5

From: PKC-ѳ is dispensable for OX40L-induced TCR-independent Treg proliferation but contributes by enabling IL-2 production from effector T-cells

Figure 5

Exogenous IL-2 rescues the Treg proliferation phenotype when added to co-cultures containing WT and PKCθ-KO not OX40-KO CD4+ T-cells. Total WT, OX40-KO and PKCθ-KO splenic CD4+ T-cells, isolated and CellTrace-labelled, were co-cultured with either WT-BMDCs or II-KO-BMDCs in the presence or absence of exogenous IL-2. After 5 days, cells were harvested and analysed by FACS. (a) Representative dot plots show the proliferating Tregs upon co-culture of CD4+ T-cells. (b) Bar graphs comparing the percentage of proliferating Tregs from OX40-KO and PKCθ-KO to WT CD4+ T-cells, in various co-culturing conditions. Values show average ± SD, *P < 0.05, **p < 0.005, and ***p < 0.0005.

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