Figure 6
From: The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
PSMP expression was up-regulated in the initial stage of DSS-induced colitis. (A) The mouse colons were separated each day during colitis induction, and on the fifth day of colitis, bloody excrement was observed. (B) The colon lengths were measured and statistically analyzed each day during the induction of colitis. (C) The body weights of the mice were measured and statistically analyzed each day during the induction of colitis. (D) The colonic tissue biopsies were stained with HE. (E) The concentrations of PSMP, IL-6, TNF-α, CCL2, IFN-γ, IL-10, and IL-12p70 in the DSS colitis tissue homogenates over time were detected by CBA. (F) The percentages of immune cells (CD45+) among the cLP cells and the percentages of myeloid-derived cells (CD11b+), granulocytes (CD11b+Ly6G+), macrophages (CD11b+F4/80+), CCR2+ myeloid-derived cells, and CD11c+ among the CD45+ immune cells over time detected by flow cytometry. (G) The time course of PSMP expression in the colonic tissue biopsies by PSMP IHC staining. Scale bar = 100 μm. Data were representative of at least two independent experiments with three mice per group. *0.01 < p < 0.05, **0.001 < p < 0.01, and ***p < 0.001.
