Figure 7
From: The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
PSMP expression was induced by LPS and MDP in CECs, and PSMP promoted the expression of CCL2 in M1 macrophages. (A–D) Primary CECs from wild type mice were stimulated with LPS, Pam3CSK4, MDP or ATP for 3 h. PSMP mRNA expression was assessed by real-time PCR. (E) During the monocyte-macrophage polarization procession, the PSMP expression in the cell culture medium was detected by CBA and CCL2 expression was detected in parallel by ELISA. (F) The CCL2 expression was detected in the medium of M0, M1 and M2 cells with and without PSMP (100 ng/mL) stimulating at the indicated time by ELISA and statistically analyzed. Data were representative of at least three independent experiments. *0.01 < p < 0.05, **0.001 < p < 0.01, and ***p < 0.001.
