Figure 2
From: Flavonoid Enhances the Glyoxalase Pathway in Cerebellar Neurons to Retain Cellular Functions
(A) Analysis of D-lactate release into extracellular space from neurons treated with MG (500 μM) and flavonoids (10 µM). Control = no MG, Vehicle = only MG. Results are means +/− SEM of multiple independent experiments performed in triplicate. #P < 0.05 (vs Control), *P < 0.05 (vs Vehicle). (B) Concentration of MG (µM/mL) in extracellular space was determined in cerebellar neurons treated with MG (500 μM) and flavonoids (10 µM). Values are means +/− standard error of samples performed in triplicate from several independent experiments. #P < 0.05 (vs Control), *P < 0.05 (vs Vehicle). (C) Concentration of GSH in cellular lysates was determined in cerebellar neurons treated with MG (500 µM) and flavonoids (10 µM). Values are means +/− standard error of samples performed in triplicate from several independent experiments. ##P < 0.01 (vs Control), *P < 0.05 (vs Vehicle), **P < 0.01 (vs Vehicle). (D) Glyoxalase 1 activity was determined in cerebellar neurons treated with MG (500 µM) and flavonoids (10 µM). Values are means +/− standard error of samples performed in triplicate from several independent experiments. #P < 0.05 (vs Control), *P < 0.05 (vs Vehicle).
