Figure 3

Putative pathway for tetranortriterpenoid biosynthesis from Azadirachta indica. Name of the enzymes catalyzing biochemical steps are shown between the reactions and expression of unigenes encoding these enzymes in leaf and fruit is shown by heatmap. Broken arrows represent putative steps involving enzymes involved in secondary modifications. AACT, acetyl CoA acetyltransferase; HMGS, 3-hydroxy-3-methylglutaryl-coenzymeA synthase; HMGR, 3-hydroxy-3-methylglutaryl-coenzymeA reductase; MVK, mevalonate kinase; PMK, phosphomevalonate kinase; PMD, phosphomevalonate decarboxylase; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; DXR, 1-deoxy-D-xylulose-5-phosphate reductoisomerase; MCT, 2-C-methyl-D-erythritol-4-phosphate cytidylyl transferase; CMK, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; MDS, 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase; HDS, 4-hydroxy-3-methylbut-2-enyldiphosphate synthase; HDR, 4-hydroxy-3-methylbut-2-enyldiphosphate reductase; IPPI, isopentenyl diphosphate isomerase; FPPS, farnesyl diphosphate synthase; SQS, squalene synthase; SQE, squalene monooxygenase/epoxidase.