Figure 5

Peptidomics of α-Gal IgE-reactive peptides. (a) IgE reactive gastric peptides were identified using peptidomics and their corresponding regions in the bovine thyroglobulin sequence were identified (uniprot.org accession number P1027). Gastric peptides of 15–20 kDa, dark box; gastric peptides of 10–15 kDa, dashed box. Dark triangles above the sequence represent N-glycosylation sites with complex type of glycans; Dark squares above the sequence represent N-glycosylation sites with high mannose type of glycan. (b) Model of α-Gal glycan carried on peptide or protein. The IgE binding part of the glycan, the Gal-α1,3-Gal epitope, is marked in the blue circle; The complex Man₃GlcNAc₂ core of the glycan, where the end of the core is attached to protein/peptide with N-glycosylation linkage to Asn-X-Thr(Ser) sequence (black arrow), is marked in the red circle. Model was created using Avogadro software v1.1.1.