Figure 6
From: Peptidomics of an in vitro digested α-Gal carrying protein revealed IgE-reactive peptides
Mapping and influence of other glycans on IgE-reactive peptides. (a) Detection of Neu5Gc on bovine thyroglobulin and the corresponding digestion products using an anti-Neu5Gc antibody. Lane 1–Bovine thyroglobulin; Lane 2–Bovine thyroglobulin peptides obtained after 60 minutes of gastric digestion; Lane 3–Porcine pepsin only as control after 60 minutes of digestion; M–Molecular weight markers. (b) Patient IgE binding to HSA-NAc-β-1,4-galactose-α-1,3-galactose, HSA-galactose-α-1,3-galactose and HSA-NAc-β-1,4-galactose as measured by ELISA.
