Figure 1 | Scientific Reports

Figure 1

From: Osteogenic Factor Runx2 Marks a Subset of Leptin Receptor-Positive Cells that Sit Atop the Bone Marrow Stromal Cell Hierarchy

Figure 1

LepR+ cells in the bone marrow cavity express Runx2. (A–H) Z-stack confocal (A,D–F and H) and confocal (B,C and G) images of thick bone sections of Runx2-GFP mice (5–6 weeks old). The images were taken from whole bone tissue (A), endosteum (B,C and F), epiphyseal cartilage (D) and bone marrow (E,G and H). Bone tissues are stained with osteocalcin (Red) (B), SOST (Red) (C), Type-2 collagen (Col2) (Red) (D), CD45 and Ter119 (Red) (E), VE-cadherin (VE-Cad) and CD31 (Red) (F), Perilipin (White) (G), and Leptin receptor (LepR) (Red) (H) antibodies. Nuclei are visualized with Hoechst 33342 (blue) and propidium iodide (PI) (Red). Arrows: Osteoblasts (B), Osteocytes (C), and LepR+ cells (H).

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