Figure 2
From: Ssu72 attenuates autoimmune arthritis via targeting of STAT3 signaling and Th17 activation
Ssu72 controls inflammatory responses in vitro. (A) NIH-3T3 cells were transfected with a control siRNA or Ssu72 siRNA and stimulated with IL-6 (20 ng/ml) for 0.5 h. Cells were used to examine the p-STAT3 Tyr705 and p-STAT3 Ser727 levels. (B) Expression levels of the Ssu72 mRNA in cells transfected with the siRNAs were measured by real-time PCR. (C) NIH-3T3 cells were transfected with the Il17a promoter construct and either the siRNA control or siRNA Ssu72 to detect luciferase activity. (D) NIH-3T3 cells were transfected with siRNAs and stimulated with IL-6 (20 ng/ml) for 0.5 h. Real-time PCR was performed to measure the expression levels of the IL-1β, IL-17A, IL-21, TBK1, and IKBKE mRNAs. The data represent the mean ± SD from three independent experiments. Statistical analyses were conducted using the nonparametric Mann-Whitney U-test (*P < 0.05, **P < 0.03, ***P < 0.01).
