Figure 3
From: Ssu72 attenuates autoimmune arthritis via targeting of STAT3 signaling and Th17 activation
Ssu72 ameliorates the development of CIA. (A) The Ssu72 overexpression or mock vector was administered systemically to mice with CIA once per week. The clinical scores and incidence of CIA were measured in these mice (***P < 0.01, n = 10). (B) Total IgG levels were measured in each group (***P < 0.01, n = 10). (C) The levels of p-STAT3 Tyr705 and p-STAT3 Ser727 in splenocytes from mice with CIA (mock or Ssu72-overexpressing) that had been stimulated with IL-6 (20 ng/ml) for 1 h were examined by western blot analysis. (D) Joint tissues from mice with CIA were stained with hematoxylin and eosin (original magnification, 40× or 200×, n = 6) and Safranin O (original magnification, 40×, n = 6). (E) Immunohistochemical staining was used to detect IL-6, IL-21, IL-17, IL-1β, TNF-α and RANKL in the synovium of mice with CIA (mock or Ssu72-overexpressing; (original magnification, 200× or 400×, n = 6). The data represent the mean ± SD from three independent experiments. Statistical analyses were conducted using the nonparametric Mann-Whitney U-test (*P < 0.05, **P < 0.03, ***P < 0.01).
