Figure 4
From: Ssu72 attenuates autoimmune arthritis via targeting of STAT3 signaling and Th17 activation
Ssu72 inhibits the progression of osteoclastogenesis. (A) TRAP expression in the synovium of mice with CIA (mock or Ssu72-overexpressing) was observed using immunohistochemical staining (original magnification, 200× or 400×, n = 6). (B and C) Bone marrow cells from mice with CIA (mock or Ssu72-overexpressing) were cultured with macrophage colony-stimulating factor (M-CSF) (10 ng/ml) and RANKL (50 ng/ml). Cells were fixed, stained for TRAP, and the number of TRAP+ cells was counted using a light microscope (original magnification 100×, n = 6). Real-time PCR was performed to measure the relative mRNA levels of osteoclastogenic markers (n = 6). The data represent the mean ± SD from three independent experiments. Statistical analyses were conducted using the nonparametric Mann-Whitney U-test (*P < 0.05, **P < 0.03, ***P < 0.01).
