Figure 2

Tissue imaging of mouse brain sections and an exogenous lipid spotted on rat brain section in positive ion mode. (A) Average mass spectra and the corresponding ion images obtained from three adjacent tissue sections using three different aspiration capillaries, respectively, in a high mass resolution mode with a mass resolution of 200,000 (at m/z 400) and a spatial resolution of 150 μm. (B) Optical image and MSI result of different amounts (from 2.6 pmol to 80.4 pmol) of [PC(24:0)+K]+ (m/z 600.4001) spotted (1 μL) on a rat brain tissue section. (C) Standard curve by plotting the amounts of each spot against the average intensities of m/z 600.4001 each spot. Error bars represent the standard deviation of the intensity of m/z 600.4001 from about 130 mass spectra in each spot.