Figure 3

F991 relieved inflammasome activation in the inflammatory tissue of DSS-induced colitis mice. (A) The level of IL-6, TNFα, IL-10 in the cultured supernatant of colonic tissue on the 2nd, 4th and 6th day were examined by ELISA. The results are shown as the mean ± SD of 4 mice per group at each time point. ns: no statistical significance, *P < 0.05, **P < 0.01, ***P < 0.001 compared with group DSS + PBS. (B) The level of active IL-1β, IL-18, pro-caspase-1, cleaved caspase-1 (p20) and ASC in the colonic tissues on the 6th day were determined by western blot. (C) The level of phosphorylated p65 (p-p65), and phosphorylated-STAT3 (p-STAT3) in the colonic tissues on the 6th day were analyzed by western blot. DSS + PBS: mice challenged with DSS and injected with PBS, DSS + F991: mice challenged with DSS and injected with F991. One representative experiment of three is displayed.