Figure 5

F991 suppressed tumorigenesis and improved the survival rate in the AOM/DSS-induced CAC model. Mice were injected i.p. with a single dose (10 mg/kg) of AOM, and then mice were given 3 cycles of 2.5% DSS administered in the drinking water for 7 days, followed by 14 days of regular water. F991(15 mg/kg) was administered through i.p. injection daily in conjunction with the DSS treatment cycles, and the same volume of PBS was administered through i.p. in F991-vehicle mice. Mice were sacrificed on day 91 after CAC induction. (A) Body weight and (B) the survival rate of the AOM/DSS-induced CAC mouse model were monitored. (C) The colon length was measured after the mice were sacrificed on day 91. (D) Colonic tumor formation is shown. (E) Tumor number was measured and the tumor load was determined by totaling the diameter of all tumors for a given animal. (F) Colon tissues were stained with H&E. Scale bars: 100 μM. The results (A–E) are shown as the mean ± SD of 10 mice per group. ns: no statistical significance, *P < 0.05 and **P < 0.01, compared with group AOM + DSS + PBS. AOM + DSS + PBS: AOM/DSS-induced CAC mice and injected with PBS, AOM + DSS + F991: AOM/DSS-induced CAC mice and injected with F991. One representative experiment of two is displayed.