Table 1 List of primary and corresponding secondary antibodies used throughout the study.
Primary Antibody | Source | Dilution | Secondary Antibody |
|---|---|---|---|
Monoclonal anti- mTOR antibody with mouse antigen, isotype IgG γ1 kappa | Millipore, 05-1592 | 1 in 200 | Goat anti- mouse IgG γ1 Alexa®594 |
Polyclonal anti- Lamp2 antibody with rabbit antigen, isotype IgG | Abgent, AP1824d | 1 in 100 | Goat anti- rabbit IgG(H + L) Alexa®488 |
Monoclonal anti- dystrophin antibody with mouse antigen, isotype IgG 2α | Iowa Hybridoma Repository MANDYS1 clone 3B7 | 1 in 200 | Goat anti- mouse IgG 2α Alexa®488 |
Monoclonal anti- Rheb antibody with rabbit antigen, isotype IgG | AbCam, ab92313 | 1 in 50 | Goat anti- rabbit IgG (H + L) Alexa®488 |
Polyclonal anti- eIF3F antibody with rabbit antigen, isotype IgG | AbCam, ab74568 | 1 in 150 | Goat anti- rabbit IgG (H + L) Alexa®488 |
Monoclonal anti- Tuberin antibody with mouse antigen, isotype IgGγ1 | AM1919b | 1 in 50 | Goat anti- mouse IgG γ1 Alexa®594 |
Lectin from Ulex europaeus Agglutinin conjugated with FITC fluorescences (UEA-I) | Sigma Aldrich, L9006 | 1 in 300 | Alexa Fluor® 488 Conjugated |
Wheat Germ Agglutinin-350 | W11263, Invitrogen | 1 in 20 | Alexa Fluor® 350 Conjugated |
