Figure 6

Salubrinal increases p-eIF2α, ATF4, GRP78, CHOP and VEGF, and reduces NFATc1 in ONFH in vivo. (a) Representative histological immunofluorescence images of femoral head from different groups (blue: DAPI, green: p-eIF2α⁺ cells, 200×, Bar = 100 μm). (b) Numbers of p-eIF2α⁺ cells were analyzed. (c) Representative images of Western blotting in three different groups in vivo. The levels of p-eIF2α (d), ATF4 (e), GRP78 (f), CHOP (g), VEGF (h), and NFATc1 (i) are shown. The asterisks (* and **) represent P < 0.05, and P < 0.01, respectively, and “ns” indicates P > 0.05 (n = 6). (f) Proposed mechanism of salubrinal’s action on improving ONFH.