Figure 5
From: Widespread alternative exon usage in clinically distinct subtypes of Invasive Ductal Carcinoma
Validation of splicing and transcriptional events by Taqman qRT-PCR in an independent patient cohort. PCR primers and Taqman probes were designed to specifically detect transcriptional events in 5 genes in an independent cohort consisting of 20 ER+ HER2− and 20 ER− HER2− patients from the MicMa cohort. Log2 expression is shown relative to normal breast RNA. PMM1 and RPL32 were used for normalization. The following events were measured: (A) The inclusion and skipping of exon chr20:34797410-34797820 in EPB41L1. (B) The inclusion and skipping of exon chr6:76608090-76608128 in MYO6. (C) An alternative start exon (chr8:80992550-80993010) and the “canonical” start exon (chr8:81083660-81083836) of TPD52. (D) The intronic start transcript of IQCG. (E) The intronic start transcript of ACOX2. Association to ER status was assed using the Wilcoxon’s rank-sum test, ***p < 0.0005, **p < 0.005, *p < 0.05.
