Figure 3
From: Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
FA uptake by both T-cell subsets is positively correlated to the T-cell proliferation. Cell Trace Violet (CTV)-labeled T-cells were cultured under stimulating conditions of anti-CD3/anti-CD28 treatment for 72 hours and assayed for FA uptake utilizing flow cytometric analysis and compared against T-cell proliferation. The top panel (A) describes how T-cell proliferation is measured using this analysis; the bottom panels compare fatty acid uptake across different extents of proliferation in both CD4+ and CD8+ T-cell subsets. In (B), the average FA-Qdot 605 fluorescence for each cycle of proliferation was plotted, and a logarithmic regression was performed to determine the correlation between numbers of T-cell division and palmitic acid uptake. Results are complied from four separate experimental wells per group and are representative of 2 experiments. Statistics are calculated as the percentage of positive population ± SEM. One-way ANOVA, followed by Tukey’s post-hoc analysis, was used to calculate significance. p<0.05*; p<0.01**; p<0.001***. Relative Fluorescence Units = (RFU).
