Figure 5
From: Fatty Acid Uptake in T Cell Subsets Using a Quantum Dot Fatty Acid Conjugate
In-vivo uptake of Fatty Acids can be detected by FA-Qdot conjugate, but not by conventional palmitic acid (PA)-BODIPY dye. Wild-type C57/Bl6 mice were injected with 10nM of either FA-Qdot 605 or PA-BODIPY conjugate. After 4 hours, T cells were isolated from the spleen and analyzed via flow cytometry. In (A), comparison of particle uptake shown as a percentage of each T cell subset as analyzed via flow cytometry; top panel (NH2) dye, middle panel (PA-BODIPY), bottom panel (FA-Qdot). In (B), panels showing the gating strategy for delineating naïve (CD62L+ CD44−) compared to activated T cells (CD62L− CD44+), as both a total of each T cell subset (top panel) and gated only on those that have taken up FA (bottom panel). Results are compiled from four animals. Statistics are calculated as the percentage of positive population ± SEM. One-way ANOVA, followed by Tukey’s post-hoc analysis, was used to calculate significance. p<0.05*; p<0.01**; p<0.001***. Relative Fluorescence Units = (RFU).
