Figure 1 | Scientific Reports

Figure 1

From: Asxl1 deficiency in embryonic fibroblasts leads to cellular senescence via impairment of the AKT-E2F pathway and Ezh2 inactivation

Figure 1

Phenotypic comparison of wild-type and homozygous-Asxl1 null littermates. (a) Wild-type and homozygous-Asxl1 null embryos at embryonic day E18.5. (b) Wild-type (left), heterozygous (middle), and homozygous-Asxl1 null embryos (right) at embryonic day E13.5. The genotyping results are represented in the upper right corner. (c) Proliferation curves of Asxl1 +/+ (solid line), Asxl1 +/− (long dotted line), and Asxl1 −/− (short dotted line) MEFs at E13.5. (d) Growth curves of Asxl1 −/−, Asxl1 +/−, and wild-type MEFs (passage 5) in complete medium with 10.0% and 0.1% FBS. Each experiment was performed in triplicate. Data shown represent the mean ± SD of three independent experiments. Data were subjected to Student’s t-test; a significant difference vs. WT-MEFs is indicated as *p < 0.05.

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