Figure 3 | Scientific Reports

Figure 3

From: Asxl1 deficiency in embryonic fibroblasts leads to cellular senescence via impairment of the AKT-E2F pathway and Ezh2 inactivation

Figure 3

Genome-wide analysis in Asxl1 −/− and Asxl1 +/+ primary MEFs (passage 5). (a,b) Distribution of functional classification of the 1,128 genes that were significantly higher (≥2-fold) in Asxl1 −/− MEFs than in Asxl1 +/+ MEFs. Pie chart (a) and bar graph (b) representation of gene ontology for genes differentially expressed in microarray analysis according to biological process. (c) Clustering analysis of genes associated with cell cycle. In total, 129 genes were analyzed. (d,e) Gene set enrichment analysis (GSEA). GSEA was performed by comparing Asxl1-responsive genes with two different gene sets associated with the cell cycle process (d; REACTOME_CELL_CYCLE) and E2F1 signaling (e; V$E2F1_Q3). (f) Validation of mRNA expression. Total RNAs were purified and reverse-transcribed. cDNA was amplified by RT-qPCR using specific primer sets for known E2F target genes (Supplementary Table 5). mRNA levels were normalized to endogenous β-actin. Data are shown as mean ± SD of three independent experiments (*p < 0.05).

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