Figure 6 | Scientific Reports

Figure 6

From: Asxl1 deficiency in embryonic fibroblasts leads to cellular senescence via impairment of the AKT-E2F pathway and Ezh2 inactivation

Figure 6

Functional correlation between Asxl1 disruption and AKT1 inhibition. (a,b) The induction of senescence by an AKT1 inhibitor. Wild-type (WT) and Asxl1-null MEFs were treated with IGF-1 alone or IGF-1 plus AKT inhibitor IV (iAKT) and subjected to senescence assays by SA-β-gal staining and SAHF formation. The numbers of β-gal stained cells (a) or cells with heterochromatin foci (b) were counted. Data are the mean ± SD of three independent experiments (*p < 0.05). (c) The effect of the AKT1 inhibitor on the subcellular localization of p27Kip1. MEFs were treated with IGF-1 alone or IGF-1 plus iAKT and subjected to immunofluorescence microscopy using anti-p27 antibody and Alexa 488-conjugated secondary antibody.

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