Figure 6
Applications of CLARITY to diverse biological systems. (a) Wildtype E10.5 mouse embryo was cleared and labeled with Tuj1 and laminin. Scale: 500 μm. (b) E12.5 Embryo from Wnt1-Cre x Rosa26/TdTomato mouse was clarified and imaged, demonstrating robust preservation of endogenous labels. Scale: 100 μm. (c) Whole E14.5 mouse embryo was stained for Tuj1 and Laminin, the antibody label removed, and the specimen restained with the same antibody. Scale: 1000 μm. (d,e) Even after two rounds of staining, the same fine structural features (arrowheads) can be easily identified in the whole sample (green box). Scale: 200 μm. (f) CLARITY can successfully clear decalcified bone (see Methods). Scale: 1000 μm. Samples in panels a-f were embedded in A1B1P4 hydrogel and cleared for 2–4 weeks at 37 °C (see Methods and detailed protocol for tissue-dependent timing). (g–k) Other whole organs can also be cleared, including the gastrointestinal tract (here, pyloric sphincter, scale: 500 μm), lung (scale: 500 μm), heart (scale: 1000 μm), kidney (scale: 100μm), and skeletal muscle (scale: 50 μm). CLARITY preserves fine structures, enabling visualization of, for example, neuromuscular junctions (arrowheads). Samples in panels (g–k) were from adult Wnt1-Cre; Rosa26-mT/mG reporter lines, embedded in A4B4P0 hydrogel and clarified for 1–2 wk at 37 °C (see detailed protocol for tissue-specific timing).
