Table 1 Mutator phenotype and genetic basis of hypermutation in CC274.

From: Evolution of the Pseudomonas aeruginosa mutational resistome in an international Cystic Fibrosis clone

Isolate IDa

ST

Mutator?

Complement with

Sequence variation in mutator genes (mutome)b

ung

mfd

mutS

sodB

mutT

sodM

mutL

mutM

oxyR

polA

AUS034

274

Yes

mutL

    

E236D

 

R631C

D61N L132P

 

D876E

AUS410

274

No

   

E25V

     

D876E

AUS411

274

No

    

E236D

  

D61N

 

D876E

AUS531

274

No

    

E236D

  

D61N

 

D876E

AUS588

274

No

   

E25V

     

D876E

AUS601

1043

Yes

mutL

S13R

  

E25V

  

P159S H288Y

 

F106L H219Y

D876E

AUS603

274

No

   

E25V

     

D876E

AUS690

274

Yes

mutS

 

Q1123H

C224R T287P

 

E236D

  

D61N

 

D876E

FQRC10

274

No

    

E236D

  

D61N

 

D876E

FQRC15

274

No

    

E236D

  

D61N

 

D876E

FQRC26

274

No

    

E236D

  

D61N

 

D876E

FQSE03

274

No

  

L374V

 

E236D

  

D61N

 

D876E

FQSE06-0403

274

No

    

E236D

  

D61N

 

D876E

FQSE06-1104

274

Yes

mutS

  

Nt814Δ4

 

E236D

  

D61N

 

D876E

FQSE06-0807

274

No

    

E236D

  

D61N

 

D876E

FQSE06-0610

274

No

    

E236D

  

D61N

 

D876E

FQSE10-0503

274

No

    

E236D

  

D61N

 

D876E

FQSE10-0106

274

No

    

E236D

  

D61N

 

D876E

FQSE10-0110

274

No

    

E236D

  

D61N

 

D876E

FQSE10-0111

274

No

    

E236D

  

D61N

 

D876E

FQSE15-0803

274

No

    

E236D

  

D61N

 

D876E

FQSE15-0906

274

No

    

E236D

  

D61N

 

D876E

FQSE15-0310

274

No

    

E236D

  

D61N

 

D876E

FQSE15-1110

1089

Yes

mutS

 

A868T

Nt814Δ4

 

E236D

  

D61N

 

D876E

FQSE24-0304

1089

Yes

mutS

  

Nt814Δ4

 

E236D

  

D61N

 

D876E

FQSE24-1005

1089

Yes

mutS

  

Nt814Δ4

 

E236D

  

D61N

 

D876E

FQSE24-0308

1089

Yes

mutS

  

Nt814Δ4

 

E236D

  

D61N

 

D876E

FQSE24-1010

1089

Yes

mutS

  

Nt814Δ4

 

E236D

  

D61N

 

D876E

PAMB148

274

No

    

E236D

L202R

 

D61N

 

D876E

  1. aIsolates are labelled according to the following format: Patient identification - MMYY isolation code in the case of sequential isolates.
  2. bSequence variations respect to those of PAO1. No mutations were found in other genes associated with mutator phenotypes, including pfpI, mutY, dnaQ, PA2583, PA2819.1, PA2819.2, radA and uvrD.