Figure 1

Analysis of Longitudinal mutDNA kinetics in MIBC. (A) Study design. 17 patients were enrolled at the NKI for mutDNA analysis whilst undergoing NAC. TUR was performed at the NKI or at regional institutions. PLS, UCP and USN were taken on one occasion before the initiation of NAC and subsequently on each chemotherapy visit, prior to definitive therapy. (B) Examples of longitudinal SNV and CNA analysis performed for each sample are shown. (C) Grid depicting mutDNA detection across all patients and time-points. The y-axis shows patients grouped by recurrence status, (right-side) and individual mutations (left-side). The x-axis shows sample-types (top) and time-points (bottom). Each cell of the grid indicates the result of a mutation analysis at that time-point (mutant-time-point analysis). White cells correspond to unavailable samples and light blue cells to samples where analysis did not detect mutDNA. Purple, yellow, red and green cells correspond to TUR, PLS, UCP and USN samples (respectively) where a mutDNA was detected. No mutations were detected in BUF (Grey). Raw AFs for the grid are provided in Supplementary Table 4.