Figure 2

CG-induced peritoneal fibroblast accumulation and proliferation is dependent on CTGF. (a) Accumulation of proliferating fibroblasts (GFP⁺PCNA⁺). Peritoneal sections at day 21 were obtained from mice treated with control IgG or FG-3019. Representative tissue sections stained with anti-GFP antibody (green)/anti-PCNA antibody (red) are shown. Bars, 50 μm. (b) Numbers of GFP⁺ cells in the peritoneum are expressed as the mean number ± SEM per HPF (n = 5 mice/group). (c) Numbers of peritoneal GFP⁺PCNA⁺ cells (proliferating fibroblasts) are expressed as mean number ± SEM per HPF. (d) Percentages of peritoneal fibroblasts that are proliferating (GFP⁺PCNA⁺ cells/total GFP⁺ cells).