Figure 7
Inhibition of CTGF suppressed TGF-β1-induced MMT in PMCs. (a) αSMA expression upon stimulation of PMCs with 5 ng/ml TGF-β1. (n = 3 cell preparations/group). (b) Validation of siRNA inhibition of CTGF. PMCs were transfected with CTGF siRNA or control siRNA, and CTGF expression in response to TGF-β1 was determined 2 hours after the stimulation. (n = 3 cell preparations/group). (c) The effect of CTGF knockdown on TGF-β1-induced αSMA expression. PMCs were transfected with control siRNA or CTGF siRNA, and then stimulated with TGF-β1 (5 ng/ml for 24 hrs). (n = 3 cell preparations/group). (d) Effect of CTGF inhibition on TGF-β1-induced αSMA expression. PMCs were preincubated with FG-3019 (10 μg/ml) or control IgG (10 μg/ml) for 1 h, and then stimulated with TGF-β1 (5 ng/ml for 24 hrs). (n = 6 cell preparations/group). (e) E-cadherin expression upon stimulation of PMCs with 5 ng/ml TGF-β1. (n = 3 cell preparations/group). (f) The effect of CTGF knockdown on TGF-β1-induced E-cadherin expression. PMCs were transfected with control siRNA or CTGF siRNA, and then stimulated with TGF-β1 (5 ng/ml for 24 hrs). (n = 3 cell preparations/group). (g) Effect of CTGF inhibition on TGF-β1-induced E-cadherin expression. PMCs were preincubated with FG-3019 (20 μg/ml) or control IgG (20 μg/ml) for 1 h, and then stimulated with TGF-β1 (5 ng/ml for 24 hrs). (n = 3 cell preparations/group). All data are expressed as mean ± SEM.
