Figure 1

Alpha diversity in whole larvae microbiota significantly decreases between 0–3 and 4–20 DPH, but significantly increases from 4–20 to 50–80 and 80–100 DPH. Non-parametric Shannon (np Shannon) diversity indexes of whole larvae and fry feces microbiota are represented through time in (a) (in a scatter plot) and (b) (in a boxplot, including significance letters of t-tests at p-value threshold of 0.05). The np Shannon diversity of other microbial niches is represented in (c). Chao diversity indexes of whole larvae and fry feces microbiota are represented through time in (d) (in a scatter plot) and (e) (in a boxplot, including significance letters of t-tests at p-value threshold of 0.05). The Chao diversity of other microbial niches is represented in (f). Note that the adult diet is not represented in this figure (neither in c nor f), as only one sample was collected from this microbial niche. Green markers represent samples taken before mucus feeding (0–3 DPH), blue markers represent samples taken during mucus feeding (4–20 DPH), yellow markers represent samples taken early after mucus feeding (50–80 DPH), and orange markers represent sample taken later after mucus feeding (80–100 DPH). Data layouts in (a and c) are best explained by logarithmic type trendlines. In (b and c), y-axis labels are the same than in (a). In (e and f), y-axis labels are the same than in (d). Since contrasted patterns of diversity were observed between 0–3 DPH to 4–20 DPH (decrease) and 50–80 DPH to 80–100 DPH (increase), the graphs in a and d are composites of two separate graphs that were merged together, to better illustrate the contrasted patterns from early to later developmental stages. The composite graphs in (a and d) were: one diversity scatter plot from 0–3 to 4–20 DPH, and another diversity scatter plot from 4–20 DPH to 50–80 and 80–100 DPH. The logarithmic trendlines were plotted independently on each composite graphs, before merging.