Figure 2

Convergence of whole larvae and fry feces microbial community towards adult-like gut microbiota. The bar charts in (a,b,c,d and e) respectively represent the relative abundances (%) of the most abundant 5 bacterial phyla, 10 bacterial classes, 10 bacterial orders, 15 bacterial families, and 15 bacterial genera in all samples, in regards to the number of DPH. In this figure, “AF” stands for “Adult feces” (or feces from the discus parents), “BDM” stands for “Breeding discus mucus”, “NBDM” stands for “Non-breeding discus mucus”, and “AD” stands for “Adult diet”. In (f), the relative abundances (%) of five OTUs from the Erysipelotrichaceae family is shown in regards to the number of DPH and to the different microbial niches studied. Note that in (f), the developmental stage from 4–20 DPH is decomposed in its five samplings at 6, 9, 13, 16, and 20 DPH to document more precisely the increased abundance of the Erysipelotrichaceae family during this key developmental stage. In (g), a Multi-Factorial Analysis (MFA) is shown including all the samples from the different niches (except the sample from Adult diet, since it distorted the representation of all the other samples). The MFA was constructed with the FactomineR package from R, using the relative abundance of the 50 most abundant OTUs in all samples. Confidence ellipses highlight differences at a 0.95 confidence threshold. PERMANOVAs between the three clusters were done on a Thetayc distance matrix, using the vegan package from R, with 10 000 permutations.