Table 2 PERMANOVAs highlight maturation of the fry fecal microbiota before 80–100 DPH. Results of the PERMANOVAs (10,000 permutations) comparing the microbial communities of the whole fry or fry fecal microbiota from different life stages with the one in their parents’ feces. Comparisons were only made between similar types of biological samples: whole fry samples compared to whole fry samples, feces samples compared to feces samples, and skin mucus samples compared to skin mucus samples. PERMANOVAs were performed with package vegan on R. “df” stands for “degrees of freedom”.
Comparison | df (residual) | F-value | p-value | ||
|---|---|---|---|---|---|
Fry | Whole fry microbiota from 0–3 DPH (before mucus feeding) | Whole fry microbiota from 4–20 DPH (during mucus feeding) | 5 | 3.2363 | 0.04762* |
Fry fecal microbiota from 50–80 DPH (after mucus feeding) | Fry fecal microbiota from 80–100 DPH (after mucus feeding) | 5 | 0.9723 | 0.4857 NS | |
Fry fecal microbiota from 50–80 DPH (after mucus feeding) | Parents’ feces | 9 | 3.4198 | 0.0121* | |
Fry fecal microbiota from 80–100 DPH (after mucus feeding) | Parents’ feces | 9 | 2.0718 | 0.07469 NS | |
Parents | Mucus microbiota when feeding fry | Mucus microbiota when not feeding fry | 10 | 1.5933 | 0.0384* |
