Figure 4
From: Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair
Core/Shell 3D printing by co-axial extrusion. (A) Schematic representation of the 3D co-axial handheld printer. (B) Schematic representation of the co-axial nozzle. (C) Picture of the cartridges dedicated to Core and Shell loading in the printer, with relative magnification of the nozzle during co-axial deposition. (D) Representative 3D rendered confocal images of Core/Shell printed sample labeled with fluorescent beads. The Shell (GelMa/HAMa plus LAP 0.1%) is shown in red channel, while the Core (GelMa/HAMa) is shown in green channel. z-stacks were acquired every 10 µm and 3D rendering was performed with NIS elements software using the Alpha-blending algorithm. A Nikon Plan Fluor 10x DIC L N1 NA0.3 objective lens was used. The panel shows the same image representative of 3D rendered of superimposed green and red channels in three different orientations.
