Figure 6
From: Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair
The Core/Shell design allows cells to proliferate. (A) Representative 3D rendered confocal images of ADSCs bioprinted samples stained with Calcein-AM (live cells, green channel) at day 1 and day 15 after printing. The Core/Shell samples were generated by labeling with fluorescent beads the Shell (GelMa/HAMa plus LAP 0.1%, cyan channel). z-stacks were acquired every 10 μm and 3D rendering was performed with NIS elements software using the Alpha-blending algorithm. A Nikon Plan Fluor 10x DIC L N1 NA0.3 objective lens was used. Images show representative 3D rendered of superimposed (merge) cyan and green channels of confocal single 2D z-stacks. (B) The graph shows the representation of the percentages of the same area occupied by cells (Cells percentage) and Shell (Shell percentage). (C) Fluorescence images of 10 μm cryosections obtained from the cryopreserved bioprinted samples. The cyan channel represents the labeled Shell, while cells have been stained with DAPI before imaging. A fluorescent Olympus IX70 inverted microscope with a SPOT Diagnostic RT-Slider camera and SPOT Diagnostic software was used. Olimpus 4X UPlanFL NA0.13, 10X CPlanFL RC NA0.3 and 20X LCPlanFL RC2 NA0.4 objective lenses were used.
