Figure 5
From: Defective Sphingosine-1-phosphate metabolism is a druggable target in Huntington’s disease
Levels of essential sphingolipids are defective in brain tissues from R6/2 mice. Lipidomic analysis by LC-MS/MS of S1P, Sph and Cer content in striatal (A–C) and cortical (D,F) tissues from manifest (11 week old) R6/2 mice and WT littermates. Values are represented as mean ± SD. N = 7–10 for each group of mice. *p < 0.05; **p < 0.001; ***p < 0.0001 (Unpaired t-test). Representative immunohistochemical micrograph (G) and dot blotting and densitometric analysis (H) of Cer content in the cortex of manifest R6/2 mice and WT littermates. Scale bar in each micrograph represents 100 µm. Data are represented as mean ± SD. N = 5 for each group of mice. **p < 0.001 (Unpaired t-test).
