Figure 2 | Scientific Reports

Figure 2

From: The LRRK2 G2385R variant is a partial loss-of-function mutation that affects synaptic vesicle trafficking through altered protein interactions

Figure 2

LRRK2 interacts with proteins involved in vesicle trafficking. (A) Immunoprecipitation of endogenous LRRK2 from adult forebrain mouse lysate shows that LRRK2 interacts with β3-Cav2.1, synapsin I and β-Actin. (B) DIV14 cortical neurons were processed for imaging purposes and stained with specific antibodies recognizing LRRK2, β3-Cav2.1, Synapsin I and F-Actin. (C) Graph shows the Pearson’s correlation coefficient to appreciate the overlap between LRRK2 and the indicated protein. (D) We isolated on streptavidin resin full-length strep-FLAG-LRRK2 and strep-FLAG-LRRK2ΔWD40 protein from N2A over-expressing cells. Interacting proteins were resolved by western-blotting. (E) We evaluated the extent of β3-Cav2.1, Synapsin I and β-Actin bound to the different LRRK2 variants. Data are expressed as optical density and normalized versus amount of precipitated LRRK2 protein. Graphs report mean ± S.E, *p < 0.05, Student’s T-test, n = 4.

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