Table 2 The total reads sequenced, the intragenic/exonic/intronic/intergenic rates, and the number of genes detected in each sample are presented here.

Sample	Total reads sequenced	Mapped	Mapped unique	Intragenic rate	Exonic rate	Intronic rate	Intergenic rate	Genes detected	Mean coverage-High	Mean coverage-Medium	Mean coverage-Low
FF3	42023726	37592844	27995882	0.948	0.884	0.065	0.052	15674	376.99	16.37	3.82
FF10	37112186	30779958	22735702	0.926	0.862	0.065	0.073	15421	371.14	12.91	3.24
FF13	18788002	16758670	13529630	0.945	0.884	0.061	0.054	14611	212.97	7.47	1.73
FF14	59651682	54662138	36210246	0.947	0.885	0.062	0.052	16031	532.51	22.87	5.98
FF15	34965998	32923072	24999524	0.948	0.886	0.062	0.052	15355	366.59	14.58	3.41
FF16	45049566	41338508	28216626	0.944	0.854	0.089	0.056	15742	395.65	15.88	3.93
FF17	42367044	38687824	26996622	0.930	0.865	0.066	0.070	15508	408.80	16.06	4.01
FF18	38247446	35096216	22061352	0.932	0.873	0.059	0.068	15612	340.66	12.77	3.01
FF20	45156828	41265828	28362946	0.938	0.872	0.066	0.062	16034	399.06	16.36	3.98
FF21	62590502	54318504	35711252	0.943	0.877	0.065	0.057	16030	506.28	21.60	5.31
NF1	46016224	40107626	28253026	0.926	0.862	0.064	0.074	16032	395.65	16.10	4.07
NF4	39105994	36621378	27604732	0.929	0.871	0.059	0.070	15517	386.04	14.99	3.64
NF7	51543864	47002456	33462460	0.937	0.875	0.062	0.063	16027	444.52	19.48	4.56
NF8	39174808	35558266	26244832	0.925	0.864	0.061	0.075	15803	381.37	14.67	3.54
NF9	44996604	40845860	29106082	0.919	0.855	0.063	0.081	15878	383.28	16.38	3.86
NF10	51037730	45634882	32393242	0.933	0.867	0.066	0.067	15735	466.67	18.78	4.39
NF11	46797824	43182836	31797826	0.938	0.874	0.063	0.062	15951	426.46	18.68	4.24

High, medium, and low mean coverage calculations are based on the top 1000, middle 1000, and bottom 1000 expressed transcripts, respectively.

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