Table 3 Validation of highly upregulated or downregulated genes using real-time quantitative PCR.

From: Genome-wide RNA-Sequencing analysis identifies a distinct fibrosis gene signature in the conjunctiva after glaucoma surgery

Gene

RT-qPCR

RNA-Seq

Fold change (FF vs NF)

p value

Fold change (FF vs NF)

p value

MYOCD

+30.22**

0.005

+11.45**

0.006

LMO3

+7.11*

0.046

+9.13**

0.003

IL-6

+2.70**

0.001

+3.04***

0.0003

RELB

+1.74*

0.017

+2.14**

0.001

PRG4

āˆ’47.62*

0.023

āˆ’49.61***

0.0006

CD34

āˆ’28.57*

0.033

āˆ’14.75**

0.003

IL-33

āˆ’27.78*

0.043

āˆ’19.90***

0.0005

MMP-10

āˆ’20.06*

0.018

āˆ’16.54***

0.0003

WISP2

āˆ’15.15*

0.027

āˆ’8.78**

0.009

COL6A6

āˆ’13.39*

0.033

āˆ’7.41***

0.0008

IGFBP5

āˆ’6.29*

0.022

āˆ’3.94**

0.002

  1. All mRNA values were normalised relative to that of GAPDH and triplicate experiments were performed for each gene. Statistically significant differences were expressed as *pā€‰<ā€‰0.05; **pā€‰<ā€‰0.01; ***pā€‰<ā€‰0.001.
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