Figure 3
From: Taxanes and platinum derivatives impair Schwann cells via distinct mechanisms
Effects of paclitaxel, cisplatin and oxaliplatin on Schwann cell morphology and MBP expression. (a) Phase (upper) and fluorescence (lower) micrographs of differentiated Schwann cells labeled with an anti-MBP antibody. Differentiated Schwann cells were treated with vehicle (0.1% DMSO), paclitaxel (0.01 μM), cisplatin (1 μM) or oxaliplatin (3 μM) for 48 h. Cisplatin and oxaliplatin reduced the number of MBP-positive Schwann cells, but did not affect cell morphology. Unlike cisplatin and oxaliplatin, paclitaxel induced morphological changes, characterized by retraction of bipolar processes and a rounded shape. Scale bar: 100 μm (25 μm in enlarged images). (b) Expression of MBP in Schwann cells 48 h after treatment with vehicle (0.1% DMSO), paclitaxel (0.001 and 0.01 μM) or cisplatin (1 and 3 μM) was analyzed by Western blotting. (Upper panels) Representative Western blot showing expression of MBP and actin. (Lower panels) Quantification of band intensity. The intensity of each band was normalized to that of actin (loading control). Each column represents the mean ± S.E.M. n = 5–8. *p < 0.05 and ***p < 0.001 vs. the vehicle-treated group. Images of the full blots are shown in Supplementary Fig. S3.
