Figure 4
From: Taxanes and platinum derivatives impair Schwann cells via distinct mechanisms
Effects of paclitaxel, cisplatin and oxaliplatin on primary cultured DRG neurons or on co-cultures of Schwann cells and DRG neurons. (a) Immunofluorescence staining of primary cultured DRG neurons for MAP2. Primary cultured DRG neurons were treated with vehicle (0.1% DMSO), paclitaxel (0.01 and 0.1 μM), cisplatin (1 and 3 μM) or oxaliplatin (3 and 10 μM) for 48 h. Only higher doses of paclitaxel (0.1 μM), cisplatin (3 μM) and oxaliplatin (10 μM) reduced the number of DRG neurons and associated dendritic trees, and shortened the neuritic processes. Scale bar: 100 μm. (b) Co-cultures were treated with vehicle (0.1% DMSO), paclitaxel (0.01 μM), cisplatin (1 μM), or oxaliplatin (3 μM) for 48 h. Scale bar: 200 μm. Each treatment reduced formation of MBP-positive myelin; however, no morphological changes were observed in MAP2-positive DRG neurons. (c) MBP- or MAP2-IR in co-cultures. Each column represents the mean ± S.E.M. n = 3. ***p < 0.001 vs. the vehicle-treated group.
