Figure 6
From: Taxanes and platinum derivatives impair Schwann cells via distinct mechanisms
Effect of paclitaxel, cisplatin and oxaliplatin on expression of p75 in primary cultured Schwann cells. Schwann cells were treated with vehicle (0.1% DMSO), paclitaxel (0.01 μM), cisplatin (1 μM) or oxaliplatin (3 μM) for 48 h. (a) Immunofluorescent staining of primary cultured Schwann cells for p75 (red) and GFAP (green). Scale bar: 100 μm. (b) p75-IR in Schwann cells. Each column represents the mean ± S.E.M. n = 3. ***p < 0.001 vs. the vehicle-treated group. Only weak p75-IR was observed in GFAP-positive Schwann cells after treatment with vehicle, cisplatin, or oxaliplatin, whereas paclitaxel increased p75-IR in GFAP-positive Schwann cells. (c) Quantitative real-time PCR analysis of p75 mRNA expression in Schwann cells. Each column represents the mean ± S.E.M. n = 3. ***p < 0.001. Treatment with paclitaxel, but not cisplatin, led to a significantly increased in expression of p75 mRNA.
