Figure 7
From: Taxanes and platinum derivatives impair Schwann cells via distinct mechanisms
Effect of paclitaxel, cisplatin and oxaliplatin on expression of galectin-3 in primary cultured Schwann cells. Schwann cells were treated with vehicle (0.1% DMSO), paclitaxel (0.01 μM) or cisplatin (1 μM) for 48 h. (a) Immunofluorescence staining of primary cultured Schwann cells with DAPI (blue) and an anti-galectin-3 antibody (green). Scale bar: 100 μm. (b) Galectin-3-IR in Schwann cells. Each column represents the mean ± S.E.M. n = 3. ***p < 0.001 vs. the vehicle-treated group. Only weak galectin-3-IR was observed in Schwann cells after treatment with vehicle or cisplatin, whereas paclitaxel increased galectin-3-IR in Schwann cells. (c) Quantitative real-time PCR analysis of galectin-3 mRNA expression in Schwann cells. Each column represents the mean ± S.E.M. n = 3. ***p < 0.001. Treatment with paclitaxel, but not cisplatin, led to a significant increase in expression of galectin-3 mRNA.
