Figure 8
From: Taxanes and platinum derivatives impair Schwann cells via distinct mechanisms
Changes in the expression of p75 and galectin-3 in sciatic nerves isolated from mice treated with paclitaxel. Paclitaxel (4 mg/kg) was injected intraperitoneally on Days 0, 3, 5, and 7. At 1 day after the last injection, mice were fixed and subjected to immunohistochemical assay. (a) Longitudinal sections of sciatic nerve isolated from saline- or paclitaxel-treated mice were co-immunolabeled for p75 (red), or MAP2, GFAP or galectin-3 (green). Scale bar: 50 μm (10 μm in enlarged images). Repeated injection of paclitaxel increased p75-IR in GFAP- or galectin-3-positive Schwann cells surrounding MAP2-positive neural axons. (b) Quantification of the intensity of fluorescence associated with MAP2, p75 or galectin-3. Each column represents the mean ± S.E.M. n = 3–5. *p < 0.05 and **p < 0.01 vs. the vehicle-treated group.
