Figure 1 | Scientific Reports

Figure 1

From: Endothelial cells by inactivation of VHL gene direct angiogenesis, not vasculogenesis via Twist1 accumulation associated with hemangioblastoma neovascularization

Figure 1

Knockdown of VHL gene expression in HUVEC cells by shRNA. The graph illustrated the schematic information of lentivirus vector pLKO.1 puro used for VHL shRNA construction (A). Western blot analysis (B) and qRT-PCR(C) analysis evaluated the knockdown efficiency of VHL gene in HUVEC cells 72 h after transduction. Statistical analysis was performed using unpaired Student’s t test, *** represent P < 0.001, **** represent P < 0.0001. The original figure of western blotting was shown in Supplementary Figure S1.

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