Figure 2 | Scientific Reports

Figure 2

From: Endothelial cells by inactivation of VHL gene direct angiogenesis, not vasculogenesis via Twist1 accumulation associated with hemangioblastoma neovascularization

Figure 2

The effect of VHL gene knockdown on proliferation and apoptosis of HUVEC cells in vitro. Knockdown of VHL gene expression significantly promoted proliferation (A) and decreased apoptosis (B) of HUVEC cells in vitro. An increase in the percentage of cells in S or G2/M phase in sh-VHL groups was observed compared to the control group (P < 0.05) (C). Western blots results showed knockdown of VHL up-regulated Twist1 expression in HUVEC cells (D). All these data were representative of three independent experiments. Statistical analysis was performed using unpaired Student’s t test, * represent P < 0.05. The original figure of western blotting was shown in Supplementary Figure S2.

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