Figure 7

Differential effects of chrysin and Ru-thio-chrysin in the modulation of thrombus formation under arterial flow conditions and haemostasis in mice. DiOC6-labelled human whole blood was treated with vehicle control [0.1% (v/v) DMSO] or 100 μM chrysin or Ru-thio-chrysin for 5 minutes prior to the perfusion over collagen-coated Vena8 biochips. Thrombus formation was monitored by capturing Z-stack fluorescent images at every 30 seconds over 10 minutes by fluorescence microscopy. The images shown (A) at different time points and traces (B) are representative of three separate experiments. (C) The mean fluorescence intensity obtained with vehicle control (V) at 10 minutes was taken as 100% to calculate the level of inhibition in chrysin (C) and Ru-thio-chrysin (Ru-tc)-treated samples. Data represent mean ± S.D. (n = 3). *Indicates significance with respect to control and ^#indicates significance with respect to the respective chrysin concentration; p values shown (***^,### p < 0.001) are as calculated by one-way ANOVA using Graphpad Prism. (D) The effects of vehicle control [0.1% (v/v) DMSO] or 25 μM of chrysin (C) or Ru-thio-chrysin (Ru-tc) in the modulation of haemostasis were analysed by tail bleeding assay in mice. Data represent ± S.D. (n = 8 mice in each group). The p values shown (*p < 0.05 and ***p < 0.001) are as calculated by non-parametric Mann-Whitney test using Graphpad Prism.