Figure 5
From: Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
Exogenous profilin 1 decreased THP-1 ALOX5 expression. (a) ALOX5 mRNA was decreased after 24 hr treatment with PFN1 (100 μM) in THP-1 cells (n = 4). ALOX5 protein was decreased after (b) 48 h (n = 3) and (c) 72 hr (n = 3) of PFN1 (100 µM) treatment on THP-1 cells determined by Western blot normalized to GAPDH. mRNA expression was determined by semi-quantitative PCR normalized to 18S. Data are mean ± SEM, *p < 0.05, significant difference from control. a-c: student’s t-test.
