Figure 3 | Scientific Reports

Figure 3

From: Absence of Tissue Inhibitor of Metalloproteinase-4 (TIMP4) ameliorates high fat diet-induced obesity in mice due to defective lipid absorption

Figure 3

Reduced adipocyte size, fibrosis and inflammation in Timp4 −/− mice on high fat diet. H&E staining (A) and adipocyte size distribution (B) in epidydimal adipose tissue of indicated groups. Picrosirius Red staining (C), and mRNA expression of collagen 1α1 (Col1α1)(Di) and collagen IIIα1 (ColIIIα1) (Dii). (E) mRNA expression of Tnfα (i), Il6 (ii), and Mcp1 (iii). (F) Immunostaining to detect macrophages in epidydimal adipose tissue (F4/80 antibody). Averaged data are presented as mean ± S.E.M and analyzed by ANOVA. *Indicates significance (p < 0.05).

Back to article page